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RFXAP antibody - ChIP grade

Anti-RFXAP antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60368

Cat. No. GTX60368

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ChIP assay

Reactivity

Human
Package
50 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ChIP assay 1-10 μg
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RFXAP (Regulatory factor X-associated protein), using the recombinant protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulatory factor X associated protein

Cellular Localization

Nucleus

Background

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RFXAP antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60368

GTX60368 ChIP assay Image

ChIP analysis of sheared chromatin from 2x10⁶ NALM cells using GTX60368 RFXAP antibody - ChIP grade. An anti-flag antibody (lane 2) was used as negative IP control. This figure shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).
Antibody amount : 5-7μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 429