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RGS2 antibody

Orthogonal
Anti-RGS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104944
Anti-RGS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104944
Anti-RGS2 antibody used in Western Blot (WB). GTX104944
Anti-RGS2 antibody used in Western Blot (WB). GTX104944
Anti-RGS2 antibody used in Western Blot (WB). GTX104944
Anti-RGS2 antibody used in Western Blot (WB). GTX104944

Cat. No. GTX104944

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RGS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RGS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951707

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulator of G protein signaling 2 , G0S8

Background

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 2 belongs to this family. The protein acts as a mediator of myeloid differentiation and may play a role in leukemogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RGS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104944

GTX104944 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using RGS2(GTX104944) antibody at 1:200 dilution.

Anti-RGS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104944

GTX104944 IHC-P Image

RGS2 antibody detects RGS2 protein at cytoplasm and nucleus in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
RGS2 antibody (GTX104944) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RGS2 antibody used in Western Blot (WB). GTX104944

GTX104944 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody (GTX104944) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RGS2 antibody used in Western Blot (WB). GTX104944

GTX104944 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody (GTX104944) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RGS2 antibody used in Western Blot (WB). GTX104944

GTX104944 WB Image

Orthogonal

22RV1 whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody (GTX104944) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)
Multiple bands were observed 26 kDa, and 24 kDa. It is possibly due to alternative splicing.
Reference: PMID: 31940362

Anti-RGS2 antibody used in Western Blot (WB). GTX104944

GTX104944 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS2 antibody (GTX104944) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 24 kDa, and 28 kDa. It is possibly due to alternative splicing.
Reference: PMID: 31940362

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169