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RGS21 antibody

Anti-RGS21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85280
Anti-RGS21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85280
Anti-RGS21 antibody used in Western Blot (WB). GTX85280
Anti-RGS21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85280

Cat. No. GTX85280

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all RGS21 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 μg/mL
ICC/IF 2.5 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

Hep G2 Cell Slide(GTX25530) , HepG2 Cell Lysate(GTX27900)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RGS21 antibody was raised against a 16 amino acid synthetic peptide near the center of human RGS21.The immunogen is located within amino acids 40 - 90 of RGS21.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulator of G protein signaling 21

Background

Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS21 is a recently identified member of this family that has been reported to be selectively expressed in subpopulations of taste bud cells and co-expressed with bitter and sweet transduction components such as alpha-gusticin, phospholipase Cbeta2, T1R2/T1R3 sweet taste receptors and T2R bitter taste receptors. Other reports indicate that RGS21 is more widely expressed. Binding assays demonstrate that RGS21 binds alpha-gusticin in a conformation-dependent manner and may do the same with T1R receptors, suggesting that RGS21 may play a role in sweet and bitter taste transduction processes.

Database

DATA IMAGES

Anti-RGS21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85280

GTX85280 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX85280 RGS21 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

Anti-RGS21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85280

GTX85280 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX85280 RGS21 antibody.
Working concentration : 2.5 μg/ml

Anti-RGS21 antibody used in Western Blot (WB). GTX85280

GTX85280 WB Image

WB analysis of HepG2 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX85280 RGS21 antibody.
Working concentration : 0.5 μg/ml

Anti-RGS21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85280

GTX85280 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX85280 RGS21 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399