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RIC8A antibody [3G3]

Overexpression
Anti-RIC8A antibody [3G3] used in IHC (Paraffin sections) (IHC-P). GTX83714
Anti-RIC8A antibody [3G3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83714
Anti-RIC8A antibody [3G3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83714
Anti-RIC8A antibody [3G3] used in Western Blot (WB). GTX83714
Anti-RIC8A antibody [3G3] used in Western Blot (WB). GTX83714

Cat. No. GTX83714

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all RIC8A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human RIC8A (NP_068751) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RIC8 guanine nucleotide exchange factor A , RIC8

Cellular Localization

Cytoplasm,Cell membrane

Background

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Database

Research Area

DATA IMAGES

Anti-RIC8A antibody [3G3] used in IHC (Paraffin sections) (IHC-P). GTX83714

GTX83714 IHC-P Image

IHC-P analysis of kidney tissue using GTX83714 RIC8A antibody [3G3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-RIC8A antibody [3G3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83714

GTX83714 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX83714 RIC8A antibody [3G3].

Anti-RIC8A antibody [3G3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83714

GTX83714 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with RIC8A plasmid using GTX83714 RIC8A antibody [3G3].

Anti-RIC8A antibody [3G3] used in Western Blot (WB). GTX83714

GTX83714 WB Image

Overexpression

WB analysis of HEK293T cells transfected with RIC8A plasmid (Right) or empty vector (Left) for 48 hrs using GTX83714 RIC8A antibody [3G3].
Loading : 5 ug per lane

Anti-RIC8A antibody [3G3] used in Western Blot (WB). GTX83714

GTX83714 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX83714 RIC8A antibody [3G3].
Loading : 10 ug per lane
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519