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RIF1 antibody

Anti-RIF1 antibody used in Western Blot (WB). GTX131889
Anti-RIF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131889
Anti-RIF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131889
Anti-RIF1 antibody used in Immunoprecipitation (IP). GTX131889
Anti-RIF1 antibody used in Western Blot (WB). GTX131889
Anti-RIF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131889

Cat. No. GTX131889

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RIF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

274 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus of human RIF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886532

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication timing regulatory factor 1

Cellular Localization

Nucleus , Telomere

Background

This gene encodes a protein that shares homology with the yeast teleomere binding protein, Rap1 interacting factor 1. This protein localizes to aberrant telomeres may be involved in DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-RIF1 antibody used in Western Blot (WB). GTX131889

GTX131889 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RIF1 antibody (GTX131889) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RIF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131889

GTX131889 ICC/IF Image

Rif1 antibody detects Rif1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rif1 protein stained by Rif1 antibody (GTX131889) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-RIF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131889

GTX131889 IHC-P Image

Rif1 antibody detects Rif1 protein at cytoplasm and nucleus in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Rif1 antibody (GTX131889) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RIF1 antibody used in Immunoprecipitation (IP). GTX131889

GTX131889 IP Image

Immunoprecipitation of Rif1 protein from HeLa whole cell extracts using 5 μg of Rif1 antibody (GTX131889).
Western blot analysis was performed using Rif1 antibody (GTX131889).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-RIF1 antibody used in Western Blot (WB). GTX131889

GTX131889 WB Image

Rif1 antibody detects Rif1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rif1 antibody (GTX131889) diluted at 1:2000.

Anti-RIF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131889

GTX131889 IHC-P Image

Rif1 antibody detects Rif1 protein at cytoplasm and nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Rif1 antibody (GTX131889) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Legartov? S et al. RNA Biol 2022; 19 (1) : 1153-1171 Early recruitment of PARP-dependent m(8)A RNA methylation at DNA lesions is subsequently accompanied by active DNA demethylation.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169