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RIM2 antibody

Anti-RIM2 antibody used in Western Blot (WB). GTX85035
Anti-RIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85035
Anti-RIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85035

Cat. No. GTX85035

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all RIM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

160 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918)

Product Note

Multiple isoforms of RIM2 are known to exist. This antibody is predicted to have no cross-reactivity to other Rim proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rim2 antibody was raised against a 17 amino acid synthetic peptide near the center of the human Rim2.The immunogen is located within amino acids 250 - 300 of Rim2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10734528

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

regulating synaptic membrane exocytosis 2 , OBOE , RAB3IP3 , RIM2

Cellular Localization

Cell membrane,Cell junction, synapse

Background

Rab3-interacting molecules (RIMs) are synaptic proteins necessary for neuronal transmission and plasticity. Rim1 and Rim2 proteins are expressed in overlapping but distinct patterns throughout the brain. While the ablation of either gene was not lethal in mice, the deletion of both resulted in postnatal mortality. This lethality is due to a defect in neurotransmitter release; synapses without RIM proteins can still release neurotransmitters but are unable to do so in response to normal Ca2+ triggers. Like Rim1, Rim 2 is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP-dependent manner. Rim2 is known to exist in multiple isoforms; this antibody should recognize most of them. This antibody is predicted to have no cross-reactivity to other Rim proteins.

Database

Research Area

DATA IMAGES

Anti-RIM2 antibody used in Western Blot (WB). GTX85035

GTX85035 WB Image

WB analysis of human brain tissue lysate using GTX85035 RIM2 antibody.
Working concentration : 1 μg/ml

Anti-RIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85035

GTX85035 IHC-P Image

IHC-P analysis of human brain tissue using GTX85035 RIM2 antibody.
Working concentration : 5 μg/ml

Anti-RIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX85035

GTX85035 IHC-P Image

IHC-P analysis of human brain tissue using GTX85035 RIM2 antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Lai CY et al. J Neurosci 2016; 36 (37) : 9722-38 Spinal Fbxo3-Dependent Fbxl2 Ubiquitination of Active Zone Protein RIM1α Mediates Neuropathic Allodynia through CaV2.2 Activation.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399