APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:20 - 1:50 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human PEBP1 (NP_002558.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphatidylethanolamine binding protein 1 , HCNP , HCNPpp , HEL-210 , HEL-S-34 , HEL-S-96 , PBP , PEBP , PEBP-1 , RKIP
Cellular Localization
Cytoplasm
Background
This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]
Database
Research Area
DATA IMAGES
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GTX54574 ICC/IF Image
ICC/IF analysis of C6 cells using GTX54574 RKIP antibody.
Blue : DAPI
Dilution : 1:100
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GTX54574 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX54574 RKIP antibody.
Blue : DAPI
Dilution : 1:100
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GTX54574 ICC/IF Image
ICC/IF analysis of L929 cells using GTX54574 RKIP antibody.
Blue : DAPI
Dilution : 1:100
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GTX54574 WB Image
WB analysis of various sample lysates using GTX54574 RKIP antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX54574 IP Image
IP analysis of MCF-7 cell lysate using GTX54574 RKIP antibody.
Antibody amount : 3μg / 150μg lysate
Dilution : 1:500
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REFERENCE
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REVIEW
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