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RND1 antibody [4F12]

Overexpression
Anti-RND1 antibody [4F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83709
Anti-RND1 antibody [4F12] used in Western Blot (WB). GTX83709
Anti-RND1 antibody [4F12] used in Western Blot (WB). GTX83709
Anti-RND1 antibody [4F12] used in Flow cytometry (FACS). GTX83709

Cat. No. GTX83709

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4F12

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($519)
See all RND1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human RND1 (NP_055285) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho family GTPase 1 , ARHS , RHO6 , RHOS

Cellular Localization

Cell membrane,Cytoplasm, cytoskeleton

Background

Lacks intrinsic GTPase activity. Has a low affinity for GDP, and constitutively binds GTP. Controls rearrangements of the actin cytoskeleton. Induces the Rac-dependent neuritic process formation in part by disruption of the cortical actin filaments. Causes the formation of many neuritic processes from the cell body with disruption of the cortical actin filaments.

Database

Research Area

DATA IMAGES

Anti-RND1 antibody [4F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83709

GTX83709 ICC/IF Image

ICC/IF analysis of HeLa cell lysate using GTX83709 RND1 antibody [4F12].
Dilution : 1:200

Anti-RND1 antibody [4F12] used in Western Blot (WB). GTX83709

GTX83709 WB Image

WB analysis of HeLa cell lysate using GTX83709 RND1 antibody [4F12].
Loading : 10 ug per lane
Dilution : 1:200

Anti-RND1 antibody [4F12] used in Western Blot (WB). GTX83709

GTX83709 WB Image

Overexpression

WB analysis of HEK293T cells transfected with RND1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83709 RND1 antibody [4F12].
Loading : 5 ug per lane

Anti-RND1 antibody [4F12] used in Flow cytometry (FACS). GTX83709

GTX83709 FACS Image

FACS analysis of HEK293T cells transfected with either RND1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83709 RND1 antibody [4F12].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519