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RNF133 antibody

Anti-RNF133 antibody used in IHC (Paraffin sections) (IHC-P). GTX117869
Anti-RNF133 antibody used in IHC (Paraffin sections) (IHC-P). GTX117869
Anti-RNF133 antibody used in Western Blot (WB). GTX117869
Anti-RNF133 antibody used in IHC (Paraffin sections) (IHC-P). GTX117869

Cat. No. GTX117869

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RNF133 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RNF133. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163354

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger protein 133

Cellular Localization

Endoplasmic reticulum membrane; Single-pass membrane protein

Background

The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene has no intron. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RNF133 antibody used in IHC (Paraffin sections) (IHC-P). GTX117869

GTX117869 IHC-P Image

RNF133 antibody detects RNF133 protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
RNF133 antibody (GTX117869) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RNF133 antibody used in IHC (Paraffin sections) (IHC-P). GTX117869

GTX117869 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RNF133(GTX117869) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RNF133 antibody used in Western Blot (WB). GTX117869

GTX117869 WB Image

Sample (30 ug of whole cell lysate)
A: K562
10% SDS PAGE
GTX117869 diluted at 1:1000

Anti-RNF133 antibody used in IHC (Paraffin sections) (IHC-P). GTX117869

GTX117869 IHC-P Image

RNF133 antibody detects RNF133 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
RNF133 antibody (GTX117869) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169