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RNF40 antibody

Anti-RNF40 antibody used in Western Blot (WB). GTX33467
Anti-RNF40 antibody used in ChIP assay (ChIP assay). GTX33467
Anti-RNF40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33467

Cat. No. GTX33467

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ChIP assay

Reactivity

 Human
Package
100 μl ($399)
See all RNF40 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
ChIP assay 1:50 - 1:200
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RNF40 (NP_055586.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger protein 40 , BRE1B , RBP95 , STARING

Cellular Localization

Nucleus

Background

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-RNF40 antibody used in Western Blot (WB). GTX33467

GTX33467 WB Image

WB analysis of various sample lysates using GTX33467 RNF40 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RNF40 antibody used in ChIP assay (ChIP assay). GTX33467

GTX33467 ChIP assay Image

ChIP analysis of HeLa cell lysate using GTX33467 RNF40 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-RNF40 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33467

GTX33467 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33467 RNF40 antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399