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RPA32 antibody [RPA2/3140R]

Anti-RPA32 antibody [RPA2/3140R] used in Western Blot (WB). GTX02702

Cat. No. GTX02702

Host

Rabbit

Clonality

Monoclonal

Clone Name

RPA2/3140R

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Observed MW

32-34 kDa. The observed M.W. is based on the following publication. PMID: 30799487

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length Replication protein A2 (RPA2) protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

replication protein A2 , REPA2 , RP-A p32 , RP-A p34 , RPA32

Cellular Localization

Nucleus

Background

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

Database

Research Area

DATA IMAGES

Anti-RPA32 antibody [RPA2/3140R] used in Western Blot (WB). GTX02702

GTX02702 WB Image

WB analysis of various samples using GTX02702 RPA32 antibody [RPA2/3140R].
Lane 1 : MCF-7 whole cell lysate
Lane 2 : T47D cell lysate

REFERENCE

There are currently no references for RPA32 antibody [RPA2/3140R] (GTX02702). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 529