GeneTex

  • Country / Location Selection

United States (US)

RPL27 antibody

Anti-RPL27 antibody used in Western Blot (WB). GTX66153
Anti-RPL27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66153
Anti-RPL27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66153
Anti-RPL27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66153
Anti-RPL27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66153

Cat. No. GTX66153

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all RPL27 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-136 of human RPL27 (NP_000979.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L27 , DBA16 , L27

Cellular Localization

Cytoplasm, cytosol,Cytoplasm

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Mar 2017]

Database

DATA IMAGES

Anti-RPL27 antibody used in Western Blot (WB). GTX66153

GTX66153 WB Image

WB analysis of various sample lysates using GTX66153 RPL27 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:3000
Loading : 25μg per lane

Anti-RPL27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66153

GTX66153 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX66153 RPL27 antibody.
Blue : DAPI
Dilution : 1:200

Anti-RPL27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66153

GTX66153 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66153 RPL27 antibody.
Blue : DAPI
Dilution : 1:100

Anti-RPL27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66153

GTX66153 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66153 RPL27 antibody.
Blue : DAPI
Dilution : 1:100

Anti-RPL27 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66153

GTX66153 ICC/IF Image

ICC/IF analysis of C6 cells using GTX66153 RPL27 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

There are currently no references for RPL27 antibody (GTX66153). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for RPL27 antibody (GTX66153). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399