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RRM1 antibody

Anti-RRM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100758
Anti-RRM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100758
Anti-RRM1 antibody used in Western Blot (WB). GTX100758
Anti-RRM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100758
Anti-RRM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100758
Anti-RRM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100758
Anti-RRM1 antibody used in Western Blot (WB). GTX100758

Cat. No. GTX100758

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RRM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

Positive Control

NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173166

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonucleotide reductase catalytic subunit M1 , R1 , RIR1 , RR1

Cellular Localization

Cytoplasm

Background

This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RRM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100758

GTX100758 ICC/IF Image

RRM1 antibody detects RRM1 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: RRM1 protein stained by RRM1 antibody (GTX100758) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-RRM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100758

GTX100758 IHC-P Image

RRM1 antibody detects RRM1 protein on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
RRM1 antibody (GTX100758) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RRM1 antibody used in Western Blot (WB). GTX100758

GTX100758 WB Image

RRM1 antibody detects RRM1 protein by Western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg Raw264.7 whole cell lysate/extract
C. 30 μg C2C12 whole cell lysate/extract
5 % SDS-PAGE
RRM1 antibody (GTX100758) dilution: 1:1000

Anti-RRM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100758

GTX100758 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RRM1(GTX100758) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RRM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100758

GTX100758 ICC/IF Image

RRM1 antibody detects RRM1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: RRM1 stained by RRM1 antibody (GTX100758) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-RRM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100758

GTX100758 IHC-P Image

RRM1 antibody detects RRM1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
RRM1 stained by RRM1 antibody (GTX100758) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-RRM1 antibody used in Western Blot (WB). GTX100758

GTX100758 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with RRM1 antibody (GTX100758) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang X et al. Oncol Lett 2021; 22 (1) : 558 Enhanced G1 arrest and apoptosis via MDM4/MDM2 double knockdown and MEK inhibition in wild-type TP53 colon and gastric cancer cells with aberrant KRAS signaling.
Application : WB
Reactivity : Human
Mu?oz-Monta?o W et al. BMC Cancer 2021; 21 (1) : 892
Application : IHC-P
Reactivity : Human
Hsieh YY et al. Cell Death Discovery 2016; 2 : 16027 Repositioning of a cyclin-dependent kinase inhibitor GW8510 as a ribonucleotide reductase M2 inhibitor to treat human colorectal cancer.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169