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RRP4 antibody [N1C3]

Anti-RRP4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117616
Anti-RRP4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117616
Anti-RRP4 antibody [N1C3] used in Western Blot (WB). GTX117616

Cat. No. GTX117616

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all RRP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721785

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

exosome component 2 , RRP4 , Rrp4p , SHRF , hRrp4p , p7

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

Component of the exosome 3'->5' exoribonuclease complex, a complex that degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions. Required for the 3'processing of the 7S pre-RNA to the mature 5.8S rRNA. Has a 3'-5' exonuclease activity.

Database

Research Area

DATA IMAGES

Anti-RRP4 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX117616

GTX117616 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RRP4(GTX117616) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RRP4 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117616

GTX117616 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RRP4(GTX117616) antibody at 1:200 dilution.

Anti-RRP4 antibody [N1C3] used in Western Blot (WB). GTX117616

GTX117616 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX117616 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169