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RSPO1 antibody

Anti-RSPO1 antibody used in Western Blot (WB). GTX85282
Anti-RSPO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85282
Anti-RSPO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85282
Anti-RSPO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85282
Anti-RSPO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85282

Cat. No. GTX85282

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all RSPO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Human Heart Tissue Lysate(GTX27916) , Human Heart Tissue Slide , Human Spleen Tissue Slide

Product Note

At least three isoforms of RSPO1 are known to exist; this antibody will detect the largest and smallest isoforms. RSPO1 antibody will not cross-react with other RSPO family members.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

RSPO1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human RSPO1.The immunogen is located within the first 50 amino acids of RSPO1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

R-spondin 1 , CRISTIN3 , RSPO

Cellular Localization

Secreted,Nucleus

Background

RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular b-catenin, thereby regulating functions mediated by b-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. At least two isoforms of RSPO1 are known to exist. RSPO1 antibody will not cross-react with RSPO family members.

Database

Research Area

DATA IMAGES

Anti-RSPO1 antibody used in Western Blot (WB). GTX85282

GTX85282 WB Image

WB analysis of human heart tissue lysate using GTX85282 RSPO1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-RSPO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85282

GTX85282 IHC-P Image

IHC-P analysis of human heart tissue using GTX85282 RSPO1 antibody.
Working concentration : 2.5 μg/ml

Anti-RSPO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85282

GTX85282 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85282 RSPO1 antibody.
Working concentration : 5 μg/ml
Green : Primary antibody
Blue : DAPI
Red : Actin

Anti-RSPO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85282

GTX85282 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85282 RSPO1 antibody.
Working concentration : 20 μg/ml

Anti-RSPO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85282

GTX85282 IHC-P Image

IHC-P analysis of human spleen tissue using GTX85282 RSPO1 antibody.
Working concentration : 5 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399