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Rad21 (S. pombe) antibody

Anti-Rad21 (S. pombe) antibody used in Western Blot (WB). GTX64147

Cat. No. GTX64147

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Schizosaccharomyces pombe
Package
50 μl ($399)
See all Rad21 products

APPLICATION

Application Note

Western blotting (1:1,000-1:4,000 dilution)

Calculated MW

68 kDa. ( Note )

Product Note

Reacts with S. pombe Rad21 protein. Not tested with other species

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

Recombinant full-size S. pombe Rad21 protein (His-tagged) expressed in E. coli

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-Rad21 (S. pombe) antibody used in Western Blot (WB). GTX64147

GTX64147 WB Image

Identification of Rad21 protein in crude extract of S. pombe with anti-Rad21 antiserum. WT cells and rad21-9xPK cells grown in YS5S to 1x107, collected, washed and broken by glass beads. Twenty ug proteins per lane were run on SDS-PAGE. First antibody at indicated dilutions in PBST plus 2.5% skim milk was incubated at 4ºC overnight. Second antibody at 1/10,000 of anti-rabbit HRP in PBST was incubated at room temperature for 1 h. Detected by ECL (X-ray film). Rad21 protein migrated at the position of ~110 kDa in SDS-PAGE although the molecular mass is 67.8 kDa. Multiple bands of Rad21 reflect multiple phosphorylation states.

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399