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Rad21 antibody

KO/KD
Anti-Rad21 antibody used in Western Blot (WB). GTX00890
Anti-Rad21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00890

Cat. No. GTX00890

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Hamster
Package
50 μg ($399)
See all Rad21 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100-1:500
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Rad21 C-terminal peptide, C-ATPGPRFHII

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD21 cohesin complex component , CDLS4 , HR21 , HRAD21 , MCD1 , NXP1 , RAD21 , SCC1 , hHR21 , Rad21

Cellular Localization

Nucleus,Chromosome,Centromere

Background

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rad21 antibody used in Western Blot (WB). GTX00890

GTX00890 WB Image

WB analysis of various samples using GTX00890 Rad21 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : NIH-3T3 whole cell lysate
Lane 4 : CHO whole cell lysate
Dilution : 1:2000
Loading : 10-20 μg

Anti-Rad21 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00890

GTX00890 ICC/IF Image

KO/KD

ICC/IF analysis of HeLa cells transfected with control or specifc hRad21 siRNA using GTX00890 Rad21 antibody.
Fixation : 4% PFA
Permeabilization : 0.5% Triton X-100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399