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Rad50 antibody

Anti-Rad50 antibody used in Western Blot (WB). GTX32832
Anti-Rad50 antibody used in Immunoprecipitation (IP). GTX32832
Anti-Rad50 antibody used in IHC (Paraffin sections) (IHC-P). GTX32832

Cat. No. GTX32832

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Rad50 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

154 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAD50 (NP_005723.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50

Cellular Localization

Nucleus

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES

Anti-Rad50 antibody used in Western Blot (WB). GTX32832

GTX32832 WB Image

WB analysis of various sample lysates using GTX32832 Rad50 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Rad50 antibody used in Immunoprecipitation (IP). GTX32832

GTX32832 IP Image

IP analysis of MCF7 cell lysate using GTX32832 Rad50 antibody.
Antibody amount : 3μg / 150μg lysate
Dilution : 1:500

Anti-Rad50 antibody used in IHC (Paraffin sections) (IHC-P). GTX32832

GTX32832 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX32832 Rad50 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399