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Rag A antibody

Anti-Rag A antibody used in IHC (Paraffin sections) (IHC-P). GTX135318
Anti-Rag A antibody used in IHC (Paraffin sections) (IHC-P). GTX135318
Anti-Rag A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135318
Anti-Rag A antibody used in Western Blot (WB). GTX135318

Cat. No. GTX135318

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rag A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Predict Reactivity

Zebrafish, Sheep, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Rag A. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2887474

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ras related GTP binding A , FIP-1 , FIP1 , RAGA

Cellular Localization

Cytoplasm,Nucleus,Lysosome

Database

Research Area

DATA IMAGES

Anti-Rag A antibody used in IHC (Paraffin sections) (IHC-P). GTX135318

GTX135318 IHC-P Image

Rag A antibody detects Rag A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Rag A stained by Rag A antibody (GTX135318) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rag A antibody used in IHC (Paraffin sections) (IHC-P). GTX135318

GTX135318 IHC-P Image

Rag A antibody detects Rag A protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Rag A stained by Rag A antibody (GTX135318) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rag A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135318

GTX135318 ICC/IF Image

Rag A antibody detects Rag A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rag A stained by Rag A antibody (GTX135318) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar= 10μm.

Anti-Rag A antibody used in Western Blot (WB). GTX135318

GTX135318 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rag A antibody (GTX135318) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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Package List Price ($)
$ 399
$ 169