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RanGAP1 antibody [N1N3]

Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700
Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700
Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700

Cat. No. GTX114700

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all RanGAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Positive Control

293T , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RanGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620615

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ran GTPase activating protein 1 , Fug1 , RANGAP , SD

Cellular Localization

Cytoplasm , Nucleus membrane; Peripheral membrane protein; Cytoplasmic side , Kinetochore

Background

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700

GTX114700 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (GTX114700) diluted at 1:2000.
The observed M.W. is based on the publication: PMID: 18706886

Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700

GTX114700 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (GTX114700) diluted at 1:2000.
The observed M.W. is based on the publication: PMID: 18706886

Anti-RanGAP1 antibody [N1N3] used in Western Blot (WB). GTX114700

GTX114700 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RanGAP1 antibody [N1N3] (GTX114700) diluted at 1:5000.
The observed M.W. is based on the publication: PMID: 18706886

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169