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RAS antibody [AT2G9]

Anti-RAS antibody [AT2G9] used in Western Blot (WB). GTX57577
Anti-RAS antibody [AT2G9] used in Flow cytometry (FACS). GTX57577
Anti-RAS antibody [AT2G9] used in Western Blot (WB). GTX57577
Anti-RAS antibody [AT2G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57577

Cat. No. GTX57577

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT2G9

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all RAS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT2G9 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NRAS protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HRas proto-oncogene, GTPase , KRAS proto-oncogene, GTPase , NRAS proto-oncogene, GTPase , C-BAS/HAS , C-H-RAS , C-HA-RAS1 , CTLO , H-RASIDX , HAMSV , HRAS1 , KRAS , KRAS2 , Ki-Ras , RASH1 , RASK2 , c-K-ras , c-Ki-ras , p21ras , C-K-RAS , CFC2 , K-RAS2A , K-RAS2B , K-RAS4A , K-RAS4B , K-Ras , KI-RAS , KRAS1 , KRAS2 , NS , NS3 , RALD , RASK2 , c-Ki-ras2 , ALPS4 , CMNS , N-ras , NCMS , NRAS1 , NS6

Cellular Localization

Golgi apparatus , Cytoplasm , Cell membrane , Golgi apparatus membrane , Cytoplasm, cytosol

Database

Research Area

DATA IMAGES

Anti-RAS antibody [AT2G9] used in Western Blot (WB). GTX57577

GTX57577 WB Image

WB analysis of 293T whole cell lysate using GTX57577 Ras antibody.
Loading : 35 μg
Dilution : 1:500~1:5000

Anti-RAS antibody [AT2G9] used in Flow cytometry (FACS). GTX57577

GTX57577 FACS Image

FACS analysis of HeLa cells using GTX57577 Ras antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

Anti-RAS antibody [AT2G9] used in Western Blot (WB). GTX57577

GTX57577 WB Image

WB analysis of various samples using GTX57577 Ras antibody.
Samples : Recombinant Human protein of NRAS, KRAS and HRAS
Loading : 200 ng

Anti-RAS antibody [AT2G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57577

GTX57577 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57577 Ras antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399