Recombinant active Human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substRates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested for its ability to enzymatically cleave these two substRates Ac-DEVD-pNA or Ac-DEVD-AFC.
Protein activity determined to be 300,000 units/mg. One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37ºC in a reaction solution containing 50 mM HEPSE (pH 7.2), 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Reconstitute with PBS, 15% Glycerol to 1 unit/μl.
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
caspase 3 , CPP32-beta , Lice , Yama
apoptotic cysteine-aspartic acid protease that may play a role in neuronal cell death regulation and other apoptotic processes [RGD, Feb 2006]