GeneTex
United States (US)

Rat Caspase 3 protein (active)

Cat No. GTX65509-pro

Species Rat
PRODUCT

Product Description

Recombinant active Human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substRates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested for its ability to enzymatically cleave these two substRates Ac-DEVD-pNA or Ac-DEVD-AFC.
APPLICATION

Application Note

Protein activity determined to be 300,000 units/mg. One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37ºC in a reaction solution containing 50 mM HEPSE (pH 7.2), 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Calculated MW

31.49 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with PBS, 15% Glycerol to 1 unit/μl.

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.

Expression System

E. coli

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

caspase 3 , CPP32-beta , Lice , Yama

Background

apoptotic cysteine-aspartic acid protease that may play a role in neuronal cell death regulation and other apoptotic processes [RGD, Feb 2006]

Database

Package List Price ($)
$ 339