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Rb antibody

IP/MS
Anti-Rb antibody used in IHC (Paraffin sections) (IHC-P). GTX100545
Anti-Rb antibody used in Immunoprecipitation (IP). GTX100545
Anti-Rb antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100545
Anti-Rb antibody used in IHC (Paraffin sections) (IHC-P). GTX100545
Anti-Rb antibody used in Western Blot (WB). GTX100545
Anti-Rb antibody used in Western Blot (WB). GTX100545

Cat. No. GTX100545

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Rb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500

Note :

WB
We would recommend using "Hot 1% SDS lysis buffer" to collect the sample lysates.
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Product Note

IP/MS validation was supported by references (PMID:30377374)

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Rb. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163170

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RB transcriptional corepressor 1 , OSRC , PPP1R130 , RB , p105-Rb , pRb , pp110

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rb antibody used in IHC (Paraffin sections) (IHC-P). GTX100545

GTX100545 IHC-P Image

Rb antibody detects Rb protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Rb stained by Rb antibody (GTX100545) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rb antibody used in Immunoprecipitation (IP). GTX100545

GTX100545 IP Image

Rb antibody immunoprecipitates retinoblastoma 1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of retinoblastoma 1 protein by 4 μg Rb antibody (GTX100545)
7.5 % SDS-PAGE
The immunoprecipitated retinoblastoma 1 protein was detected by Rb antibody (GTX100545) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Rb antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100545

GTX100545 ICC/IF Image

Rb antibody detects Rb protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rb stained by Rb antibody (GTX100545) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-Rb antibody used in IHC (Paraffin sections) (IHC-P). GTX100545

GTX100545 IHC-P Image

Rb antibody detects Rb protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Rb antibody (GTX100545) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Rb antibody used in Western Blot (WB). GTX100545

GTX100545 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Rb antibody (GTX100545) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rb antibody used in Western Blot (WB). GTX100545

GTX100545 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rb antibody (GTX100545) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Cheng TC et al. J Food Drug Anal 2021; 29 (4) : 622-637 Curcumin-induced antitumor effects on triple-negative breast cancer patient-derived xenograft tumor mice through inhibiting salt-induced kinase-3 protein.
Application : WB
Reactivity : Human
Liang ML et al. Cancers (Basel) 2020; 12 (12) Abemaciclib, A Selective CDK4/6 Inhibitor, Restricts the Growth of Pediatric Ependymomas.
Application : WB
Reactivity : Human
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Chen YJ et al. Gynecol Oncol 2013; 131 (3) : 734-43 The synthetic flavonoid WYC02-9 inhibits cervical cancer cell migration/invasion and angiogenesis via MAPK14 signaling.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Rb antibody Cat. No. GTX100545

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Rb antibody used in Western Blot (WB). GTX100545
Date : Anonymous submitted on 15-Jul-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : CL1-0 cells, CL1-5 cells
Amount used : 30μg
Blocking : 3% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane1:CL1-5 cells, Lane2:CL1-0 cells
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169