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Recoverin antibody [6a55 cd6]

Anti-Recoverin antibody [6a55 cd6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79211
Anti-Recoverin antibody [6a55 cd6] used in Western Blot (WB). GTX79211
Anti-Recoverin antibody [6a55 cd6] used in IHC (Paraffin sections) (IHC-P). GTX79211
Anti-Recoverin antibody [6a55 cd6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79211
Anti-Recoverin antibody [6a55 cd6] used in IHC (Paraffin sections) (IHC-P). GTX79211
Anti-Recoverin antibody [6a55 cd6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79211

Cat. No. GTX79211

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6a55 cd6

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Rat, Sheep, Bovine
Package
100 μg ($399)
See all Recoverin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:1000
ICC/IF 1:10-1:200
IHC-P 1:10-1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Proprietary Immunogen.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11179867

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

recoverin , RCV1

Background

This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Recoverin antibody [6a55 cd6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79211

GTX79211 ICC/IF Image

ICC/IF analysis of C6 cells using GTX79211 Recoverin antibody [6a55 cd6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-Recoverin antibody [6a55 cd6] used in Western Blot (WB). GTX79211

GTX79211 WB Image

WB analysis of 25 ug of rat eye (lane 1), rat brain (lane 2) and B-3 (lane 3) cell lysates using GTX79211 Recoverin antibody [6a55 cd6].
Dilution : 1:500

Anti-Recoverin antibody [6a55 cd6] used in IHC (Paraffin sections) (IHC-P). GTX79211

GTX79211 IHC-P Image

IHC-P analysis of human hepatocarcinoma tissue using GTX79211 Recoverin antibody [6a55 cd6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:50

Anti-Recoverin antibody [6a55 cd6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79211

GTX79211 ICC/IF Image

ICC/IF analysis of L6 cells using GTX79211 Recoverin antibody [6a55 cd6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-Recoverin antibody [6a55 cd6] used in IHC (Paraffin sections) (IHC-P). GTX79211

GTX79211 IHC-P Image

IHC-P analysis of rat liver tissue using GTX79211 Recoverin antibody [6a55 cd6].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

Anti-Recoverin antibody [6a55 cd6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79211

GTX79211 ICC/IF Image

ICC/IF analysis of B-3 cells using GTX79211 Recoverin antibody [6a55 cd6]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399