Rho GDI alpha antibody [1F2]

Cat. No. GTX84859

Host	Mouse
Clonality	Monoclonal
Clone Name	1F2
Isotype	IgG1
Application	WB, FACS
Reactivity	Human, Rat, Dog, Monkey
Package	100 μl ($519)

See all Rho GDI alpha products

Datasheet
Document

View product citations for antibody GTX84859 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
FACS	1:100

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ARHGDIA (NP_004300) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GDP dissociation inhibitor alpha , GDIA1 , HEL-S-47e , NPHS8 , RHOGDI , RHOGDI-1

Cellular Localization

Cytoplasm

Background

Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.

Database

Gene ID: 396 ARHGDIA
UniProt: P52565 ARHGDIA

Research Area

DATA IMAGES

GTX84859 WB Image

WB analysis of wild type and Rho GDI alpha knock out 293T cell lysate(10μg per lane) using GTX84859 Rho GDI alpha antibody [1F2].
Dilution : 1:500

GTX84859 FACS Image

FACS analysis of HEK293T cells transfected with either Rho GDI alpha plasmid(Red) or empty vector control plasmid(Blue) using GTX84859 Rho GDI alpha antibody [1F2].

GTX84859 FACS Image

FACS analysis of HeLa cells using GTX84859 Rho GDI alpha antibody [1F2].
Red : Primary antibody
Blue : Negative control antibody

GTX84859 FACS Image

FACS analysis of Jurkat cells using GTX84859 Rho GDI alpha antibody [1F2].
Red : Primary antibody
Blue : Negative control antibody

GTX84859 WB Image

WB analysis of HEK293T cells transfected with Rho GDI alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84859 Rho GDI alpha antibody [1F2].
Loading : 5 ug per lane

GTX84859 WB Image

WB analysis of various cell lines using GTX84859 Rho GDI alpha antibody [1F2].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

Rho GDI alpha antibody [1F2]

Cat. No. GTX84859

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84859 WB Image

GTX84859 FACS Image

GTX84859 FACS Image

GTX84859 FACS Image

GTX84859 WB Image

GTX84859 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.