Rho GDI alpha antibody [N1C3]

Name: Rho GDI alpha antibody [N1C3]
Brand: GeneTex
SKU: GTX101428
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Rho GDI alpha antibody [N1C3] used in Western Blot (WB). GTX101428

Cat. No. GTX101428

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Rho GDI alpha products

Datasheet
Document

View product citations for antibody GTX101428 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rho GDI alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241259

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GDP dissociation inhibitor alpha , GDIA1 , HEL-S-47e , NPHS8 , RHOGDI , RHOGDI-1

Cellular Localization

Cytoplasm

Background

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM]

Database

Gene ID: 396 ARHGDIA
UniProt: P52565 ARHGDIA

Research Area

DATA IMAGES

GTX101428 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Rho GDI alpha antibody [N1C3] (GTX101428) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101428 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX101428 diluted at 1:10000

REFERENCE

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REVIEW