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Ribosome binding protein 1 antibody [C3], C-term

KO/KD
Anti-Ribosome binding protein 1 antibody [C3], C-term used in Western Blot (WB). GTX101844
Anti-Ribosome binding protein 1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101844
Anti-Ribosome binding protein 1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101844
Anti-Ribosome binding protein 1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101844
Anti-Ribosome binding protein 1 antibody [C3], C-term used in Western Blot (WB). GTX101844

Cat. No. GTX101844

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Ribosome binding protein 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Positive Control

NIH-3T3 , HepG2

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ribosome binding protein 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241286

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosome binding protein 1 , ES/130 , ES130 , RRp , hES

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type III membrane protein

Background

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ribosome binding protein 1 antibody [C3], C-term used in Western Blot (WB). GTX101844

GTX101844 WB Image

KO/KD

Ribosome binding protein 1 antibody validation by siRNA knock-down.
Upperpanel: Ribosome binding protein 1 antibody GTX101844
Lower panel: Vinculin antibody (GTX109749)
A. 30 μg HepG2 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of RRBP1 siRNA#1-transfected HepG2 cells
C. 30 μg whole cell lysate/extract of RRBP1 siRNA#2-transfected HepG2 cells
5% SDS-PAGE
Ribosome binding protein 1 antibody (GTX101844) dilution: 1:500
Vinculin antibody (GTX109749) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ribosome binding protein 1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101844

GTX101844 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Ribosome binding protein 1(GTX101844) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-Ribosome binding protein 1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101844

GTX101844 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Ribosome binding protein 1(GTX101844) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ribosome binding protein 1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101844

GTX101844 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Ribosome binding protein 1(GTX101844) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Anti-Ribosome binding protein 1 antibody [C3], C-term used in Western Blot (WB). GTX101844

GTX101844 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
5% SDS PAGE
GTX101844 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ravi A et al. Eur Respir J 2019; (Epub) Neutrophilic inflammation in asthma and defective epithelial translational control.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169