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Rubicon antibody

Overexpression
Anti-Rubicon antibody used in Western Blot (WB). GTX129096
Anti-Rubicon antibody used in Immunoprecipitation (IP). GTX129096
Anti-Rubicon antibody used in Western Blot (WB). GTX129096
Anti-Rubicon antibody used in Western Blot (WB). GTX129096

Cat. No. GTX129096

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Rubicon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

109 kDa. ( Note )

Positive Control

MDA-MB-231 , NT2D1 , human Rubicon-transfected 293T cells

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Rubicon. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885892

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

rubicon autophagy regulator , KIAA0226 , RUBICON , SCAR15

Background

Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity.

Database

Research Area

DATA IMAGES

Anti-Rubicon antibody used in Western Blot (WB). GTX129096

GTX129096 WB Image

Rubicon antibody detects Rubicon protein by western blot analysis.
A. 30 μg MDA-MB-231 whole cell extract
5 % SDS-PAGE
Rubicon antibody (GTX129096) dilution: 1:1000

Anti-Rubicon antibody used in Immunoprecipitation (IP). GTX129096

GTX129096 IP Image

Rubicon antibody immunoprecipitates Rubicon protein in IP experiments.
IP samples: whole cell extract of Rubicon-transfected 293T cells
A. 30 μg 293T whole cell extract of Rubicon-protein expressing cell
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Rubicon protein by 4 μg Rubicon antibody (GTX129096)
5 % SDS-PAGE
The immunoprecipitated Rubicon protein was detected by Rubicon antibody (GTX129096) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-Rubicon antibody used in Western Blot (WB). GTX129096

GTX129096 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rubicon antibody (GTX129096) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rubicon antibody used in Western Blot (WB). GTX129096

GTX129096 WB Image

Overexpression

Rubicon antibody detects Rubicon protein by western blot analysis.
A. 30 ug 293T whole cell extract
B. 30 whole cell extract of human Rubicon-transfected 293T cells
5 % SDS-PAGE
Rubicon antibody (GTX129096) dilution: 1:20000

REFERENCE

Application Reference

Wu MY et al. EMBO Mol Med 2023; 15 (12) : e17815 Enhancement of efferocytosis through biased FPR2 signaling attenuates intestinal inflammation.
Quach C et al. Nat Commun 2019; 10 (1) : 5681 A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169