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S Tag antibody [GT727]

Anti-S Tag antibody [GT727] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629074
Anti-S Tag antibody [GT727] used in Western Blot (WB). GTX629074

Cat. No. GTX629074

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT727

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all S Tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

S-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to S Tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888092

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-S Tag antibody [GT727] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629074

GTX629074 ICC/IF Image

S Tag antibody [GT727] detects S Tag protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: S Tag stained by S Tag antibody [GT727] (GTX629074) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-S Tag antibody [GT727] used in Western Blot (WB). GTX629074

GTX629074 WB Image

S Tag antibody [GT727] detects S protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of S-tagged protein expressing 293T cells
12% SDS-PAGE
S Tag antibody [GT727] (GTX629074) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations