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S100 beta antibody [S100B/1706R]

Anti-S100 beta antibody [S100B/1706R] used in IHC (Paraffin sections) (IHC-P). GTX34998
Anti-S100 beta antibody [S100B/1706R] used in Protein Array (Protein Array). GTX34998

Cat. No. GTX34998

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 S100B/1706R

Isotype

 IgG

Application

 WB, IHC-P, Protein Array

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μg ($529)
See all S100 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1-2μg/ml for 30 minutes at RT
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Product Note

S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0).

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human S100B protein

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_2887747

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NEF , S100 , S100 calcium binding protein B , S100B , S100beta , S100 beta

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-S100 beta antibody [S100B/1706R] used in IHC (Paraffin sections) (IHC-P). GTX34998

GTX34998 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX34998 S100 beta antibody [S100B/1706R].

Anti-S100 beta antibody [S100B/1706R] used in Protein Array (Protein Array). GTX34998

GTX34998 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using S100B-Monospecific Recombinant Rabbit Monoclonal Antibody (S100B/1706R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

Application Reference

Bronzuoli MR et al. Mol Autism 2018; 9 : 66 Neuroglia in the autistic brain: evidence from a preclinical model.
Application : WB
Reactivity : Rat
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529