PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
|
Not tested in other applications.
Calculated MW
Positive Control
Spleen: A significant number of inflammatory cells should show a strong S100A12 immunostaining while the majority of cells remain completely negative.
Negative Control
Colon: All epithelial and muscular cells must not show any S100A12 immunostaining. Few scattered S100A12 positive inflammatory cells can occur (mostly within blood vessels)
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human S100A12 fragment
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
S100 calcium binding protein A12 , CAAF1 , CAGC , CGRP , ENRAGE , MRP-6 , MRP6 , p6
Cellular Localization
Secreted , Cytoplasm , Cytoplasm, cytoskeleton , Cell membrane
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity. [provided by RefSeq, Nov 2014]
Database
Research Area
DATA IMAGES
![Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449](/templates/default/js/lightbox/images/loading.gif "Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449")
|
GTX04449 IHC-P Image
IHC-P analysis of human colorectal adenocarcinoma tissue section using GTX04449 S100A12 antibody [MSVA-812M] HistoMAX™.
|
|
|
GTX04449 IHC-P Image
IHC-P analysis of human spleen tissue section using GTX04449 S100A12 antibody [MSVA-812M] HistoMAX™.
S100A12 immunostaining is seen in granulocytes of mainly the red pulp.
|
|
|
GTX04449 IHC-P Image
IHC-P analysis of human stomach Gastric adenocarcinoma tissue section using GTX04449 S100A12 antibody [MSVA-812M] HistoMAX™.
Stomach Gastric adenocarcinoma densly infiltrated by S100A12 positive granulocytes.
|
REFERENCE
There are currently no references for S100A12 antibody [MSVA-812M] HistoMAX™ (GTX04449). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for S100A12 antibody [MSVA-812M] HistoMAX™ (GTX04449). Be the first to share your experience with this product.
![Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449](/upload/website/prouct_img/small/GTX04449/GTX04449_20230728_IHC-P_108_23072722_548.webp "Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449")
![Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449](/upload/website/prouct_img/small/GTX04449/GTX04449_20230728_IHC-P_238_23072722_810.webp "Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449")
![Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449](/upload/website/prouct_img/small/GTX04449/GTX04449_20230728_IHC-P_368_23072723_220.webp "Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449")
![Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449](/upload/website/prouct_img/normal/GTX04449/GTX04449_20230728_IHC-P_108_23072722_548.webp "Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449")
![Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449](/upload/website/prouct_img/normal/GTX04449/GTX04449_20230728_IHC-P_238_23072722_810.webp "Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449")
![Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449](/upload/website/prouct_img/normal/GTX04449/GTX04449_20230728_IHC-P_368_23072723_220.webp "Anti-S100A12 antibody [MSVA-812M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04449")