*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
1-2μg/ml
ICC/IF
1-2μg/ml
IHC-P
1-2μg/ml for 30 minutes at RT
FACS
1-2μg/10⁶ cells
Protein Array
Assay dependent
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A4 Mouse Monoclonal Antibody (S100A4/1481). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX34999 FACS Image
FACS analysis of A549 cells using GTX34999 S100A4 antibody [S100A4/1481]. Blue : Primary antibody Red : Isotype control
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GTX34999 WB Image
WB analysis of HeLa cell lysate using GTX34999 S100A4 antibody [S100A4/1481].
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GTX34999 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX34999 S100A4 antibody [S100A4/1481]. Green : Primary antibody Red : nucleus
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GTX34999 FACS Image
FACS analysis of T98G cells using GTX34999 S100A4 antibody [S100A4/1481]. Blue : Primary antibody Red : Isotype control
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GTX34999 IHC-P Image
IHC-P analysis of human placenta tissue using GTX34999 S100A4 antibody [S100A4/1481].
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GTX34999 WB Image
WB analysis of HeLa, A549, and A375 cell lysates using GTX34999 S100A4 antibody [S100A4/1481].