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SAP130 antibody [N1N2], N-term

Anti-SAP130 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX122554
Anti-SAP130 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122554
Anti-SAP130 antibody [N1N2], N-term used in Western Blot (WB). GTX122554
Anti-SAP130 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX122554

Cat. No. GTX122554

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SF3B3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Rat, Zebrafish, Drosophila, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SAP130. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177245

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

splicing factor 3b subunit 3 , RSE1 , SAP130 , SF3b130 , STAF130

Cellular Localization

Nucleus

Background

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SAP130 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX122554

GTX122554 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SAP130(GTX122554) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SAP130 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122554

GTX122554 ICC/IF Image

SAP130 antibody [N1N2], N-term detects SAP130 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SAP130 protein stained by SAP130 antibody [N1N2], N-term (GTX122554) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SAP130 antibody [N1N2], N-term used in Western Blot (WB). GTX122554

GTX122554 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
5% SDS PAGE
GTX122554 diluted at 1:1000

Anti-SAP130 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX122554

GTX122554 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using SAP130(GTX122554) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kim JW et al. The American Journal of Pathology 2018; (Epub) Spliceosome-associated protein 130 exacerbates alcohol-induced liver injury by inducing NLRP3 inflammasome–mediated IL1β in mice.
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169