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SARM1 antibody

KO/KD Orthogonal
Anti-SARM1 antibody used in Western Blot (WB). GTX131411
Anti-SARM1 antibody used in Western Blot (WB). GTX131411
Anti-SARM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131411

Cat. No. GTX131411

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all SARM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Product Note

Knockdown/Knockout validation was supported by references (PMID:36055989)

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SARM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886480

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sterile alpha and TIR motif containing 1 , MyD88-5 , SAMD2 , SARM

Cellular Localization

Cytoplasm

Background

Involved in innate immnune response. Acts as a negative regulator of TICAM1/TRIF-dependent Toll-like receptor signaling by inhibiting induction of TLR3- and TLR4-dependent genes. Specifically blocks TICAM1/TRIF-dependent transcription-factor activation and gene induction, without affecting the MYD88-dependent pathway or non-TLR signaling. Negative regulator of NF-kappa-B and IRF activation.

Database

Research Area

DATA IMAGES

Anti-SARM1 antibody used in Western Blot (WB). GTX131411

GTX131411 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody (GTX131411) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARM1 antibody used in Western Blot (WB). GTX131411

GTX131411 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SARM1 antibody (GTX131411) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131411

GTX131411 ICC/IF Image

SARM1 antibody detects SARM1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARM1 stained by SARM1 antibody (GTX131411) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Xiang L et al. Cell Death Dis 2022; 13 (7) : 638 SARM1 deletion in parvalbumin neurons is associated with autism-like behaviors in mice.
Jin L et al. Cell Death Dis 2022; 13 (9) : 759 Astrocytic SARM1 promotes neuroinflammation and axonal demyelination in experimental autoimmune encephalomyelitis through inhibiting GDNF signaling.
Application : WB
Reactivity : Mouse
Sur M et al. Cell Death Discov 2018; 4 : 114 Sarm1 induction and accompanying inflammatory response mediates age-dependent susceptibility to rotenone-induced neurotoxicity.
Application : WB
Reactivity : Drosophila
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169