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SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody

Anti-SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody used in Western Blot (WB). GTX135470

Cat. No. GTX135470

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) 3CLpro (nsp5) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) 3CLpro (nsp5). The exact sequence is proprietary.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2909854

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody used in Western Blot (WB). GTX135470

GTX135470 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) 3CLpro (nsp5) antibody (GTX135470) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lin X et al. PLoS Pathog 2023; 19 (1) : e1011128 Unconventional secretion of unglycosylated ORF8 is critical for the cytokine storm during SARS-CoV-2 infection.
Application : WB
Shih PC et al. ACS Pharmacol Transl Sci 2022; 5 (6) : 400-412 Development of Ultrapure and Potent Tannic Acids as a Pan-coronal Antiviral Therapeutic.
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169