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SARS-CoV-2 (COVID-19) Envelope antibody [HL1443]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] used in Western Blot (WB). GTX636915
Anti-SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636915

Cat. No. GTX636915

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1443

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) Envelop products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of SARS-CoV-2 (COVID-19) Envelope. (Strain:Wuhan-hu-1)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] used in Western Blot (WB). GTX636915

GTX636915 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] (GTX636915) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636915

GTX636915 ICC/IF Image

SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] detects SARS-CoV-2 (COVID-19) Envelope protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Envelope stained by SARS-CoV-2 (COVID-19) Envelope antibody [HL1443] (GTX636915) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

K Miura et al. J Virol 2023; Distinct motifs in the E protein are required for SARS-CoV-2 virus particle formation and lysosomal deacidification in host cells
Application : WB
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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations