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SBP2 antibody, Internal

KO/KD
Anti-SBP2 antibody, Internal used in Western Blot (WB). GTX89253
Anti-SBP2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89253

Cat. No. GTX89253

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all SBP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
ICC/IF Assay dependent
IHC-P 3μg/ml
IP Assay dependent

Note :

IHC-P
Human Testis shows granular cytoplasm staining in Leydig cells.
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89253-PEP).

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KERQERKQRLQEN, from the internal region of the protein sequence according to NP_076982.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SECIS binding protein 2 , SBP2

Background

The protein encoded by this gene is one of the essential components of the machinery involved in co-translational insertion of selenocysteine (Sec) into selenoproteins. Sec is encoded by the UGA codon, which normally signals translation termination. The recoding of UGA as Sec codon requires a Sec insertion sequence (SECIS) element; present in the 3' untranslated regions of eukaryotic selenoprotein mRNAs. This protein specifically binds to the SECIS element, which is stimulated by a Sec-specific translation elongation factor. Mutations in this gene have been associated with reduction in enzymatic activity of type II iodothyronine deiodinase (a selenoprotein) and abnormal thyroid hormone metabolism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-SBP2 antibody, Internal used in Western Blot (WB). GTX89253

GTX89253 WB Image

KO/KD

WB analysis of HFF cell lysate with B) 100nM siRNA or A) control siRNA using GTX89253 SBP2 antibody, Internal.

Anti-SBP2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89253

GTX89253 IHC-P Image

IHC-P analysis of human testis using GTX89253 SBP2 antibody, Internal.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 3μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399