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SCAP antibody [C2C3], C-term

Anti-SCAP antibody [C2C3], C-term used in Western Blot (WB). GTX109474
Anti-SCAP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109474
Anti-SCAP antibody [C2C3], C-term used in Western Blot (WB). GTX109474
Anti-SCAP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109474

Cat. No. GTX109474

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SCAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

Positive Control

HepG2 , MCF-7

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SCAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951816

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SREBF chaperone

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein , Golgi apparatus membrane; Multi-pass membrane protein , Cytoplasmic vesicle , COPII-coated vesicle membrane; Multi-pass membrane protein

Background

This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SCAP antibody [C2C3], C-term used in Western Blot (WB). GTX109474

GTX109474 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SCAP antibody [C2C3], C-term (GTX109474) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SCAP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109474

GTX109474 IHC-P Image

SREBF chaperone protein antibody [C2C3], C-term detects SREBF chaperone protein protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
SREBF chaperone protein antibody [C2C3], C-term (GTX109474) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SCAP antibody [C2C3], C-term used in Western Blot (WB). GTX109474

GTX109474 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
5% SDS PAGE
GTX109474 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SCAP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109474

GTX109474 IHC-P Image

SREBF chaperone protein antibody [C2C3], C-term detects SREBF chaperone protein protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
SREBF chaperone protein antibody [C2C3], C-term (GTX109474) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169