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SCAP antibody

Anti-SCAP antibody used in IHC (Paraffin sections) (IHC-P). GTX03796
Anti-SCAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03796
Anti-SCAP antibody used in Western Blot (WB). GTX03796
Anti-SCAP antibody used in Flow cytometry (FACS). GTX03796
Anti-SCAP antibody used in Western Blot (WB). GTX03796
Anti-SCAP antibody used in Flow cytometry (FACS). GTX03796

Cat. No. GTX03796

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
400 μl ($429)
See all SCAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50-1:100
FACS 1:25
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This SCAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-632 amino acids from the Central region of human SCAP.

Purification

Protein A purified followed by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SREBF chaperone

Cellular Localization

Endoplasmic reticulum membrane , Golgi apparatus membrane

Background

This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-SCAP antibody used in IHC (Paraffin sections) (IHC-P). GTX03796

GTX03796 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX03796 SCAP antibody.

Anti-SCAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03796

GTX03796 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed HeLa cells using GTX03796 SCAP antibody.
Green : Primary antibody
Blue : Hoechst 33342
Permeabilization : 0.2% Triton X-100, 30 min
Dilution : 1:100

Anti-SCAP antibody used in Western Blot (WB). GTX03796

GTX03796 WB Image

WB analysis of PC-3 cell lysate using GTX03796 SCAP antibody.
Loading : 35 μg
Dilution : 1:1000

Anti-SCAP antibody used in Flow cytometry (FACS). GTX03796

GTX03796 FACS Image

FACS analysis of 2% paraformaldehyde-fixed K562 cells using GTX03796 SCAP antibody. Acquisition of >10, 000 events was performed.
Green : Primary antibody
Blue : Isotype control
Permeabilization : 90% methanol for 10 min
Dilution : 1:25

Anti-SCAP antibody used in Western Blot (WB). GTX03796

GTX03796 WB Image

WB analysis of various sample lysates using GTX03796 SCAP antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : HT-29 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : LNCap whole cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse liver tissue lysate
Lane 7 : Rat heart tissue lysate
Loading : 20 μg per lane
Dilution : 1:2000

Anti-SCAP antibody used in Flow cytometry (FACS). GTX03796

GTX03796 FACS Image

FACS analysis of 2% paraformaldehyde-fixed K562 cells using GTX03796 SCAP antibody. Acquisition of >10, 000 events was performed.
Green : Primary antibody
Blue : Isotype control
Permeabilization : 90% methanol for 10 min
Dilution : 1:25

REFERENCE

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Package List Price ($)
$ 429