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SCN1B antibody

Anti-SCN1B antibody used in IHC (Frozen sections) (IHC-Fr). GTX16973
Anti-SCN1B antibody used in Live cell imaging (LCI). GTX16973
Anti-SCN1B antibody used in Western Blot (WB). GTX16973

Cat. No. GTX16973

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, LCI

Reactivity

 Mouse, Rat
Package
50 μl ($639)
See all SCN1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Predict Reactivity

Human(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide CKRRSETTAETFTE, corresponding to amino acid residues 43-56 (Extracellular, N-terminus) of voltage-gated Na+ channels (Accession  : Q00954).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sodium voltage-gated channel beta subunit 1

Cellular Localization

Cell membrane

Background

has voltage sensitive sodium channel activity when coexpressed with alpha subunits; plays a role in initiation and propogation of the action potential [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-SCN1B antibody used in IHC (Frozen sections) (IHC-Fr). GTX16973

GTX16973 IHC-Fr Image

IHC-Fr analysis of rat DRG tissue using GTX16973 SCN1B antibody.
Panel A : NaVβ1 (red) appears in the cell bodies of the DRG neurons.
Panel B : Nuclear staining using DAPI (blue).
Panel C : Merged image of A and B.

Anti-SCN1B antibody used in Live cell imaging (LCI). GTX16973

GTX16973 LCI Image

Live cell imaging analysis of live and intact differentiated PC12 cells using GTX16973 SCN1B antibody. PC12 differentiation was induced by  Native mouse NGF 2.5S protein (>95%).
Red : Primary antibody
Blue : DAPI for nuclear staining
Dilution : 1:50

Anti-SCN1B antibody used in Western Blot (WB). GTX16973

GTX16973 WB Image

WB analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat skeletal muscle (lanes 3 and 6) tissue lysates using GTX16973 SCN1B antibody preincubated with or without immunogen peptide.
Dilution : 1:400

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639