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SCUBE2 antibody

Anti-SCUBE2 antibody used in Western Blot (WB). GTX85173
Anti-SCUBE2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85173
Anti-SCUBE2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85173

Cat. No. GTX85173

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human, Rat
Package
100 μg ($399)
See all SCUBE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
ICC/IF 20 μg/mL
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

Daudi Cell Lysate(GTX23951)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

SCUBE2 antibody was raised against a 17 amino acid synthetic peptide near the center of human SCUBE2.The immunogen is located within amino acids 480 - 530 of SCUBE2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10724347

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal peptide, CUB domain and EGF like domain containing 2 , CEGB1 , CEGF1 , CEGP1 , scube/You

Cellular Localization

Secreted

Background

SCUBE2 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and are expressed in embryonic neuroectoderm and vascular epithelium. SCUBE2 is a close homolog to SCUBE1 and can form a heterodimeric complex with SCUBE1. SCUBE2 is thought to participate in the Hedgehog signaling pathway by specifically interacting with Sonic Hedgehog (SHH) and the receptor PTCH1, enhancing the SHH signaling activity in the raft microdomains of the plasma membrane, and may thus play important roles in organ development and tumor progression.

Database

Research Area

DATA IMAGES

Anti-SCUBE2 antibody used in Western Blot (WB). GTX85173

GTX85173 WB Image

WB analysis of Daudi cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX85173 SCUBE2 antibody.
Working concentration : 1 μg/ml

Anti-SCUBE2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85173

GTX85173 ICC/IF Image

ICC/IF analysis of Daudi cells using GTX85173 SCUBE2 antibody.
Working concentration : 20 μg/ml

Anti-SCUBE2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85173

GTX85173 ICC/IF Image

ICC/IF analysis of Daudi cells using GTX85173 SCUBE2 antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Chen JH et al. Exp Cell Res 2018; (Epub) Upregulated SCUBE2 expression in breast cancer stem cells enhances triple negative breast cancer aggression through modulation of notch signaling and epithelial-to-mesenchymal transition.
Application : WB
Reactivity : Human
Lin YC et al. Arterioscler Thromb Vasc Biol 2018; (Epub) Inhibition of Endothelial SCUBE2 (Signal Peptide-CUB-EGF Domain-Containing Protein 2), a Novel VEGFR2 (Vascular Endothelial Growth Factor Receptor 2) Coreceptor, Suppresses Tumor Angiogenesis.
Application : WB
Reactivity : Human
Lin YC et al. Arterioscler Thromb Vasc Biol 2016; (Epub) Endothelial SCUBE2 Interacts With VEGFR2 and Regulates VEGF-Induced Angiogenesis.
Application : WB, ICC/IF, FACS
Reactivity : Human
Lin YC et al. J Cell Sci 2014; 127 (Pt 1) : 85-100 Tumor suppressor SCUBE2 inhibits breast-cancer cell migration and invasion through the reversal of epithelial-mesenchymal transition.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399