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SCYL3 antibody [4F10]

Overexpression
Anti-SCYL3 antibody [4F10] used in Western Blot (WB). GTX83676
Anti-SCYL3 antibody [4F10] used in Western Blot (WB). GTX83676
Anti-SCYL3 antibody [4F10] used in IHC (Paraffin sections) (IHC-P). GTX83676
Anti-SCYL3 antibody [4F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83676

Cat. No. GTX83676

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4F10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all SCYL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human SCYL3 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SCY1 like pseudokinase 3 , PACE-1 , PACE1

Cellular Localization

Cytoplasm,Golgi apparatus

Background

May play a role in regulating cell adhesion/migration complexes in migrating cells.

Database

Research Area

DATA IMAGES

Anti-SCYL3 antibody [4F10] used in Western Blot (WB). GTX83676

GTX83676 WB Image

Overexpression

WB analysis of HEK293T cells transfected with SCYL3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83676 SCYL3 antibody [4F10].
Loading : 5 ug per lane

Anti-SCYL3 antibody [4F10] used in Western Blot (WB). GTX83676

GTX83676 WB Image

WB analysis of various cell lines using GTX83676 SCYL3 antibody [4F10].
Loading : 35 ug per lane

Anti-SCYL3 antibody [4F10] used in IHC (Paraffin sections) (IHC-P). GTX83676

GTX83676 IHC-P Image

IHC-P analysis of pancreas tissue using GTX83676 SCYL3 antibody [4F10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-SCYL3 antibody [4F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83676

GTX83676 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with SCYL3 plasmid using GTX83676 SCYL3 antibody [4F10].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519