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SDHAF1 antibody, C-term

Anti-SDHAF1 antibody, C-term used in Western Blot (WB). GTX88354
Anti-SDHAF1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX88354

Cat. No. GTX88354

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all SDHAF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88354-PEP).

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-HDSTGAPETRPDGR, from the C Terminus of the protein sequence according to NP_001036096.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

succinate dehydrogenase complex assembly factor 1 , LYRM8

Cellular Localization

Mitochondrion matrix

Background

The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency).[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-SDHAF1 antibody, C-term used in Western Blot (WB). GTX88354

GTX88354 WB Image

WB analysis of human cerebellum lysate using GTX88354 SDHAF1 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-SDHAF1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX88354

GTX88354 IHC-P Image

IHC-P analysis of paraffin embedded human placenta using GTX88354 SDHAF1 antibody, C-term.
Antigen retrieval : Steamed antigen retrieval with citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399