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SEC61B antibody

Anti-SEC61B antibody used in Immunoprecipitation (IP). GTX129852
Anti-SEC61B antibody used in Western Blot (WB). GTX129852
Anti-SEC61B antibody used in Western Blot (WB). GTX129852

Cat. No. GTX129852

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SEC61B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Positive Control

mouse liver , 293T , A431 , HeLa , HepG2

Predict Reactivity

Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.77 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SEC61B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886109

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sec61 translocon beta subunit

Cellular Localization

Endoplasmic reticulum membrane; Single-pass membrane protein

Background

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SEC61B antibody used in Immunoprecipitation (IP). GTX129852

GTX129852 IP Image

Immunoprecipitation of SEC61B protein from HepG2 whole cell extracts using 5 μg of SEC61B antibody (GTX129852).
Western blot analysis was performed using SEC61B antibody (GTX129852).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SEC61B antibody used in Western Blot (WB). GTX129852

GTX129852 WB Image

SEC61B antibody detects SEC61B protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15 % SDS-PAGE, and the membrane was blotted with SEC61B antibody (GTX129852) at a dilution of 1:1000.

Anti-SEC61B antibody used in Western Blot (WB). GTX129852

GTX129852 WB Image

SEC61B antibody detects SEC61B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SEC61B antibody (GTX129852) diluted at a dilution of 1:1000.

REFERENCE

Application Reference

Chen MK et al. J Biol Chem 2019; 294 (21) : 8516-8528 H2O2 induces nuclear transport of the receptor tyrosine kinase c-MET in breast cancer cells via a membrane-bound retrograde trafficking mechanism.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169