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SEPHS2 antibody

Anti-SEPHS2 antibody used in Western Blot (WB). GTX103766
Anti-SEPHS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103766
Anti-SEPHS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103766

Cat. No. GTX103766

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SEPHS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog, Guinea pig, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SEPHS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951858

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

selenophosphate synthetase 2 , SPS2

Background

This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop. Alternatively spliced transcripts have been identified, but their biological validity has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SEPHS2 antibody used in Western Blot (WB). GTX103766

GTX103766 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
SEPHS2 antibody
GTX103766 diluted at 1:1000

Anti-SEPHS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103766

GTX103766 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using SEPHS2(GTX103766) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SEPHS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103766

GTX103766 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using SEPHS2(GTX103766) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169