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SERCA3 antibody [N1N2], N-term

Orthogonal
Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381
Anti-SERCA3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102381
Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381

Cat. No. GTX102381

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SERCA3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

Positive Control

BCL-1

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SERCA3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166236

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 3 , SERCA3

Cellular Localization

Nucleus membrane , Endoplasmic reticulum membrane , Sarcoplasmic reticulum membrane

Background

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381

GTX102381 WB Image

Sample (30 ug of whole cell lysate)
A: BCL-1
5% SDS PAGE
GTX102381 diluted at 1:5000

Anti-SERCA3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102381

GTX102381 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SERCA3(GTX102381) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381

GTX102381 WB Image

Orthogonal

Jurkat whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SERCA3 antibody [N1N2], N-term (GTX102381) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chun-Chin Chen et al. J Exp Med 2021; 218 (8) Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity is required for V(D)J recombination.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169