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SFRP4 antibody

Anti-SFRP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33493
Anti-SFRP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX33493
Anti-SFRP4 antibody used in Western Blot (WB). GTX33493

Cat. No. GTX33493

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all SFRP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 19-346 of human SFRP4 (NP_003005.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secreted frizzled related protein 4 , FRP-4 , FRPHE , FRZB-2 , PYL , sFRP-4

Cellular Localization

Secreted

Background

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-SFRP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33493

GTX33493 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33493 SFRP4 antibody.
Blue : DAPI

Anti-SFRP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX33493

GTX33493 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX33493 SFRP4 antibody.
Dilution : 1:100

Anti-SFRP4 antibody used in Western Blot (WB). GTX33493

GTX33493 WB Image

WB analysis of various sample lysates using GTX33493 SFRP4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399