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SGCE antibody [N1C1]

Anti-SGCE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116453
Anti-SGCE antibody [N1C1] used in Western Blot (WB). GTX116453
Anti-SGCE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116453

Cat. No. GTX116453

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all SGCE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human SGCE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624279

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sarcoglycan epsilon , DYT11 , ESG , epsilon-SG

Cellular Localization

Cell membrane , sarcolemma; Single-pass membrane protein (Potential) , Cytoplasm , cytoskeleton

Background

This gene encodes a member of the sarcoglycan family. Sarcoglycans are transmembrane components in the dystrophin-glycoprotein complex which help stabilize the muscle fiber membranes and link the muscle cytoskeleton to the extracellular matrix. Mutations in this gene have been associated with myoclonus-dystonia syndrome. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-SGCE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116453

GTX116453 IHC-P Image

SGCE antibody [N1C1] detects SGCE protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
SGCE antibody [N1C1] (GTX116453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SGCE antibody [N1C1] used in Western Blot (WB). GTX116453

GTX116453 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX116453 diluted at 1:1000

Anti-SGCE antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116453

GTX116453 IHC-P Image

SGCE antibody [N1C1] detects SGCE protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
SGCE antibody [N1C1] (GTX116453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Estrada-Mena FJ et al. Neurosci Lett 2017; 658 : 142-149 Effects of (-)-epicatechin on frontal cortex DAPC and dysbindin of the mdx mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169