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SH3BP1 antibody

Anti-SH3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107693
Anti-SH3BP1 antibody used in Western Blot (WB). GTX107693
Anti-SH3BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107693

Cat. No. GTX107693

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all SH3BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

HeLa , Jurkat

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SH3BP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241326

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH3 domain binding protein 1 , ARHGAP43

Background

Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac.

Database

Research Area

DATA IMAGES

Anti-SH3BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107693

GTX107693 IHC-P Image

SH3BP1 antibody detects SH3BP1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
SH3BP1 antibody (GTX107693) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SH3BP1 antibody used in Western Blot (WB). GTX107693

GTX107693 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: JurKat
7.5% SDS PAGE
GTX107693 diluted at 1:1000

Anti-SH3BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107693

GTX107693 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using SH3BP1(GTX107693) antibody at 1:200 dilution.

REFERENCE

Application Reference

Graziano BR et al. J Cell Biol 2017; A module for Rac temporal signal integration revealed with optogenetics.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169