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SHARP1 antibody

Anti-SHARP1 antibody used in Western Blot (WB). GTX04346
Anti-SHARP1 antibody used in Flow cytometry (FACS). GTX04346
Anti-SHARP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04346
Anti-SHARP1 antibody used in Western Blot (WB). GTX04346
Anti-SHARP1 antibody used in Western Blot (WB). GTX04346

Cat. No. GTX04346

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all SHARP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human BHLH3.

Purification

Protein A purified followed by peptide affinity purification

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

basic helix-loop-helix family member e41 , BHLHB3 , DEC2 , SHARP1 , hDEC2

Cellular Localization

Nucleus

Background

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-SHARP1 antibody used in Western Blot (WB). GTX04346

GTX04346 WB Image

WB analysis of mouse brain tissue lysates using GTX04346 SHARP1 antibody.
Loading : 20 μg
Dilution : 1:2000

Anti-SHARP1 antibody used in Flow cytometry (FACS). GTX04346

GTX04346 FACS Image

FACS analysis of NCI-H460 cells using GTX04346 SHARP1 antibody.
Green : Primary antibody
Blue : negative control cell

Anti-SHARP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04346

GTX04346 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed MCF-7 cells using GTX04346 SHARP1 antibody.
Green : Primary antibody
Red : Actin
Blue : DAPI
Permeabilization : 0.1 %Triton X-100, min
Dilution : 1:25

Anti-SHARP1 antibody used in Western Blot (WB). GTX04346

GTX04346 WB Image

WB analysis of human brain tissue lysates using GTX04346 SHARP1 antibody.
Loading : 20 μg
Dilution : 1:1000

Anti-SHARP1 antibody used in Western Blot (WB). GTX04346

GTX04346 WB Image

WB analysis of RD whole cell lysates using GTX04346 SHARP1 antibody.
Loading : 35 μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 399